CJC-1295 DAC (5mg)

DESCRIPTION

CJC-1295 DAC Peptide

CJC-1295 DAC is a peptide that researchers posit may function similarly to growth hormone-releasing hormone (GHRH), potentially increasing the endogenous production of growth hormone (GH) within the organism. The CJC-1295 DAC peptide is a synthetic 29 amino acid analog of GHRH.⁽¹⁾ It is the shortest functional analog of GHRH that still has the potential capacity to trigger GH release from the somatotroph cells in the pituitary gland. Moreover, 4 of the original 29 amino acids in this fragment are substituted in CJC-1295 DAC, to possibly improve the pharmacokinetics of the peptide and prolong its half-life.

The DAC component is a biochemical complex that may further increase the half-life of the peptide. This phenomenon might be ascribed to the alleged capability of the DAC to bind to proteins present in plasma. More precisely, the DAC component appears to entail the attachment of a lysine derivative, identified as N-epsilon-3-maleimidopropionamide, to the C terminus of CJC-1295 DAC. Integrating this modified amino acid sequence with the DAC component might conceivably enhance the pharmacokinetics of CJC-1295 DAC, extending its half-life to approximately 8 days. Simultaneously, it appears to maintain a significant affinity for GHRH receptors, similar to the affinity exhibited by CJC-1295 without DAC.⁽²⁾

This does not imply that CJC without DAC has no relevance or use in present day research. For example, CJC-1295 without DAC & Ipamorelin blend has been studied for its suggested synergistic potential, as combining GHRH analogs with peptides that appear to activate the ghrelin receptors in the pituitary gland. It is also posited to result in greater GH-synthesis response from the somatotroph cells.⁽³⁾

Overview

CJC-1295 DAC is recognized by many names: CJC-1295, CJC-1295 with DAC, DAC:GRF, long-acting GHRH analog, and synthetic GHRH analog. Theoretically, as the peptide is considered a releasing factor for growth hormone, CJC-1295 DAC has been studied for its potential role in:

• The reduction of fat mass by using fat cells as an energy source. It may lead to increased muscle mass via the promotion of protein synthesis.
• Since growth hormone is considered to promote bone growth and improved joint and connective tissue, CJC-1295 DAC peptide may potentially improve bone mass and thereby reduce the risk of damage.
• Studies have suggested that GHRH may support the centers in the nervous system for sleep, and potentially this action may be mirrored by analogs like CJC-1295 DAC.⁽⁴⁾

Chemical Makeup

Molecular Formula: C₁₅₂H₂₅₂N₄₄O₄₂
Molecular Weight: 3367.95 g/mol
Other Known Titles: Tetrasubsituted GRF 1-29 with DAC

Research and Clinical Studies

CJC-1295 DAC Peptide Mechanism of Action

Researchers conducted two clinical studies in 2006 to examine the action of CJC-1295 DAC. In the first study, CJC-1295 DAC or a placebo was presented in one of four ascending concentrations. In the second study, CJC-1295 DAC was presented repeatedly at a single concentration. According to the results, after the introduction of CJC-1295 DAC, there appeared to be an increase in GH and insulin-like growth factor-1 (IGF-I) levels among the research models.⁽⁵⁾CJC-1295 DAC is thought to elevate levels of IGF-1 by increasing growth hormone production, which in turn may bind to receptors on liver cells, potentially triggering a cascade of intracellular signaling processes. This binding might activate the Janus kinase-signal transducer and activator of the transcription (JAK-STAT) signaling pathway. Subsequently, the activated STAT proteins might migrate to the nucleus, where they may attach to specific DNA sequences considered to be response elements, potentially resulting in the transcription of the IGF-I gene. It is theorized that the IGF-I produced in liver cells may be transported to various target tissues. Additionally, it is believed that many tissues possess GH receptors, which, upon activation, may lead to the production of IGF-I within the tissues themselves. IGF-I is considered a potent hormone that may play a key role in promoting growth, suggesting it mediates many growth and anabolic effects of growth hormone. It is hypothesized to encourage cell growth and proliferation, as well as the enlargement and strengthening of tissues and organs, possibly aiding in protein synthesis and cellular expansion. Preliminary exposure to CJC-1295 DAC in experimental models has been suggested to significantly affect average growth hormone levels, with studies reporting an apparent increase of 2- to 10-fold for 6 days or possibly longer. Moreover, CJC-1295 DAC has been suggested to lead to dependent increases in average IGF-I levels by 1.5- to 3-fold for about 9–11 days, with suggestions that IGF-I levels may remain high for at least two weeks in experimental models. Following repeated exposure to CJC-1295 DAC, average IGF-I levels appear to remain elevated above baseline for up to 28 days. Notably, data suggests a cumulative effect following repeated exposure of the compound.⁽⁵⁾

In 2006, another group of scientists assessed the GH pulsatility after a single occurrence of CJC-1295 DAC. They found out that there appeared to be an increase of about 50% in mean GH secretion and IGF-I levels after a single presentation of CJC-1295 DAC.⁽⁶⁾ Researchers have suggested that the peptide might contribute to an increase in peak growth hormone levels by as much as 7.5 times in the models studied.⁽⁶⁾ It seems that CJC-1295 DAC may interact with certain binding sites on the growth hormone-releasing hormone (GHRH) receptor protein. This interaction may lead to changes in the receptor’s structure, potentially triggering a series of molecular processes. This interaction is believed to stimulate specific intracellular signaling proteins, often referred to as G-proteins.⁽⁷⁾ Upon activation, these proteins may promote the production of secondary messengers, such as cyclic adenosine monophosphate (cAMP) or inositol trisphosphate (IP3), which are considered to play crucial roles in cellular signaling pathways.⁽⁸⁾ Secondary messengers, including cAMP, are thought to activate protein kinases, which are enzymes considered capable of modifying specific proteins. These kinases are considered able to regulate cellular functions by phosphorylating transcription regulators, the proteins responsible for controlling gene expression. Once phosphorylated, these transcription regulators are speculated to move into the nucleus of somatotroph cells, where they might influence the genes involved in the production of growth hormone. This intricate cascade of events highlights the potential of CJC-1295 DAC to modulate growth hormone levels through a complex network of molecular interactions.

Additional animal studies were conducted to evaluate the potential of CJC-1295 DAC. One study evaluated murine models presented with either the peptide or a placebo. The researchers concluded that exposing the murine models daily CJC-1295 DAC might completely normalize growth. Another finding was that CJC-1295 DAC presented every 2 or 3 days appeared to produce intermediate results, indicating a probable interval-dependent action.⁽⁹⁾ Furthermore, this study suggests that CJC-1295 DAC might potentially impact body composition, seemingly by increasing muscle tissue hypertrophy while not impacting, or even possibly reducing, fat tissue levels. The murine models in this study appeared to have a deletion of the GHRH gene (referred to as GHRHKO); observations suggested that CJC-1295 DAC may boost GH synthesis, leading to a beneficial alteration in body composition. Exposure to CJC-1295 DAC in these GHRHKO murine models appeared to preserve normal levels of lean mass, unlike in models that were not exposed and exhibited suboptimal lean mass levels. Furthermore, the amount of subcutaneous fat mass stayed consistent with control levels in all groups associated with the peptide, whereas GHRHKO murine models without CJC-1295 DAC exposure exhibited signs of increased fat levels. This indicates that CJC-1295 DAC might positively affect muscle and bone structure without promoting an increase in fat accumulation. Additionally, the study noted a possible increase in pituitary RNA and GH mRNA levels following CJC-1295 DAC exposure, suggesting an enhanced presence of somatotroph cells—those believed to produce growth hormone in the pituitary gland. The authors commented that “CJC-1295 caused an increase in total pituitary RNA and GH mRNA, suggesting that proliferation of somatotroph cells had occurred, as confirmed by immunohistochemistry images.” ⁽⁹⁾